The role of the NADH-dependent nitrite reductase, Nir, from Escherichia coli in fermentative ammonification.
Identifieur interne : 000328 ( Main/Exploration ); précédent : 000327; suivant : 000329The role of the NADH-dependent nitrite reductase, Nir, from Escherichia coli in fermentative ammonification.
Auteurs : Xiaoguang Wang [États-Unis] ; Denis Tamiev [États-Unis] ; Jagannathan Alagurajan [États-Unis] ; Alan A. Dispirito [États-Unis] ; Gregory J. Phillips [États-Unis] ; Mark S. Hargrove [États-Unis]Source :
- Archives of microbiology [ 1432-072X ] ; 2019.
Descripteurs français
- KwdFr :
- Anaérobiose (MeSH), Composés d'ammonium (métabolisme), Escherichia coli (enzymologie), Escherichia coli (génétique), Fermentation (MeSH), Nitrite reductase (NAD(P)H) (génétique), Nitrite reductase (NAD(P)H) (métabolisme), Nitrite reductases (génétique), Nitrite reductases (métabolisme), Nitrites (métabolisme).
- MESH :
- enzymologie : Escherichia coli.
- génétique : Escherichia coli, Nitrite reductase (NAD(P)H), Nitrite reductases.
- métabolisme : Composés d'ammonium, Nitrite reductase (NAD(P)H), Nitrite reductases, Nitrites.
- Anaérobiose, Fermentation.
English descriptors
- KwdEn :
- Ammonium Compounds (metabolism), Anaerobiosis (MeSH), Escherichia coli (enzymology), Escherichia coli (genetics), Fermentation (MeSH), Nitrite Reductase (NAD(P)H) (genetics), Nitrite Reductase (NAD(P)H) (metabolism), Nitrite Reductases (genetics), Nitrite Reductases (metabolism), Nitrites (metabolism).
- MESH :
- chemical , genetics : Nitrite Reductase (NAD(P)H), Nitrite Reductases.
- chemical , metabolism : Ammonium Compounds, Nitrite Reductase (NAD(P)H), Nitrite Reductases, Nitrites.
- enzymology : Escherichia coli.
- genetics : Escherichia coli.
- Anaerobiosis, Fermentation.
Abstract
Nitrate and nitrite reduction are of paramount importance for nitrogen assimilation and anaerobic metabolism, and understanding the specific roles of each participating reductase is necessary to describe the biochemical balance that dictates cellular responses to their environments. The soluble, cytoplasmic siroheme NADH-nitrite reductase (Nir) in Escherichia coli is necessary for nitrate/nitrite assimilation but has also been reported to either "detoxify" nitrite, or to carry out fermentative ammonification in support of anaerobic catabolism. Theoretically, nitrite detoxification would be important for anaerobic growth on nitrate, during which excess nitrite would be reduced to ammonium. Fermentative ammonification by Nir would be important for maximization of non-respiratory ATP production during anaerobic growth in the presence of nitrite. Experiments reported here were designed to test the potential role of Nir in fermentative ammonification directly by growing E. coli along with mutant strains lacking Nir or the respiratory nitrite reductase (Nrf) under anaerobic conditions in defined media while monitoring nitrogen utilization and fermentation metabolites. To focus on the role of Nir in fermentative ammonification, pH control was used in most experiments to eliminate nitrite toxicity due to nitric acid formation. Our results demonstrate that Nir confers a significant benefit during fermentative growth that reflects fermentative ammonification rather than detoxification. We conclude that fermentative ammonification by Nir allows for the energetically favorable fermentation of glucose to formate and acetate. These results and conclusions are discussed in light of the roles of Nir in other bacteria and in plants.
DOI: 10.1007/s00203-018-1590-3
PubMed: 30406295
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Dispirito</name><affiliation wicri:level="4"><nlm:affiliation>Roy J Carver Department of Biochemistry Biophysics and Molecular Biology, Iowa State University, 4116 Molecular Biology Building, Ames, IA, 50011, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Roy J Carver Department of Biochemistry Biophysics and Molecular Biology, Iowa State University, 4116 Molecular Biology Building, Ames, IA, 50011</wicri:regionArea><orgName type="university">Université d'État de l'Iowa</orgName><placeName><settlement type="city">Ames (Iowa)</settlement><region type="state">Iowa</region></placeName></affiliation></author><author><name sortKey="Phillips, Gregory J" sort="Phillips, Gregory J" uniqKey="Phillips G" first="Gregory J" last="Phillips">Gregory J. 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Hargrove</name><affiliation wicri:level="4"><nlm:affiliation>Roy J Carver Department of Biochemistry Biophysics and Molecular Biology, Iowa State University, 4116 Molecular Biology Building, Ames, IA, 50011, USA. msh@iastate.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Roy J Carver Department of Biochemistry Biophysics and Molecular Biology, Iowa State University, 4116 Molecular Biology Building, Ames, IA, 50011</wicri:regionArea><orgName type="university">Université d'État de l'Iowa</orgName><placeName><settlement type="city">Ames (Iowa)</settlement><region type="state">Iowa</region></placeName></affiliation></author></analytic><series><title level="j">Archives of microbiology</title><idno type="eISSN">1432-072X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ammonium Compounds (metabolism)</term><term>Anaerobiosis (MeSH)</term><term>Escherichia coli (enzymology)</term><term>Escherichia coli (genetics)</term><term>Fermentation (MeSH)</term><term>Nitrite Reductase (NAD(P)H) (genetics)</term><term>Nitrite Reductase (NAD(P)H) (metabolism)</term><term>Nitrite Reductases (genetics)</term><term>Nitrite Reductases (metabolism)</term><term>Nitrites (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anaérobiose (MeSH)</term><term>Composés d'ammonium (métabolisme)</term><term>Escherichia coli (enzymologie)</term><term>Escherichia coli (génétique)</term><term>Fermentation (MeSH)</term><term>Nitrite reductase (NAD(P)H) (génétique)</term><term>Nitrite reductase (NAD(P)H) (métabolisme)</term><term>Nitrite reductases (génétique)</term><term>Nitrite reductases (métabolisme)</term><term>Nitrites (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nitrite Reductase (NAD(P)H)</term><term>Nitrite Reductases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ammonium Compounds</term><term>Nitrite Reductase (NAD(P)H)</term><term>Nitrite Reductases</term><term>Nitrites</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term><term>Nitrite reductase (NAD(P)H)</term><term>Nitrite reductases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Composés d'ammonium</term><term>Nitrite reductase (NAD(P)H)</term><term>Nitrite reductases</term><term>Nitrites</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Anaerobiosis</term><term>Fermentation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Anaérobiose</term><term>Fermentation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nitrate and nitrite reduction are of paramount importance for nitrogen assimilation and anaerobic metabolism, and understanding the specific roles of each participating reductase is necessary to describe the biochemical balance that dictates cellular responses to their environments. The soluble, cytoplasmic siroheme NADH-nitrite reductase (Nir) in Escherichia coli is necessary for nitrate/nitrite assimilation but has also been reported to either "detoxify" nitrite, or to carry out fermentative ammonification in support of anaerobic catabolism. Theoretically, nitrite detoxification would be important for anaerobic growth on nitrate, during which excess nitrite would be reduced to ammonium. Fermentative ammonification by Nir would be important for maximization of non-respiratory ATP production during anaerobic growth in the presence of nitrite. Experiments reported here were designed to test the potential role of Nir in fermentative ammonification directly by growing E. coli along with mutant strains lacking Nir or the respiratory nitrite reductase (Nrf) under anaerobic conditions in defined media while monitoring nitrogen utilization and fermentation metabolites. To focus on the role of Nir in fermentative ammonification, pH control was used in most experiments to eliminate nitrite toxicity due to nitric acid formation. Our results demonstrate that Nir confers a significant benefit during fermentative growth that reflects fermentative ammonification rather than detoxification. We conclude that fermentative ammonification by Nir allows for the energetically favorable fermentation of glucose to formate and acetate. These results and conclusions are discussed in light of the roles of Nir in other bacteria and in plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">30406295</PMID><DateCompleted><Year>2019</Year><Month>05</Month><Day>31</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>31</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-072X</ISSN><JournalIssue CitedMedium="Internet"><Volume>201</Volume><Issue>4</Issue><PubDate><Year>2019</Year><Month>May</Month></PubDate></JournalIssue><Title>Archives of microbiology</Title><ISOAbbreviation>Arch Microbiol</ISOAbbreviation></Journal><ArticleTitle>The role of the NADH-dependent nitrite reductase, Nir, from Escherichia coli in fermentative ammonification.</ArticleTitle><Pagination><MedlinePgn>519-530</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00203-018-1590-3</ELocationID><Abstract><AbstractText>Nitrate and nitrite reduction are of paramount importance for nitrogen assimilation and anaerobic metabolism, and understanding the specific roles of each participating reductase is necessary to describe the biochemical balance that dictates cellular responses to their environments. The soluble, cytoplasmic siroheme NADH-nitrite reductase (Nir) in Escherichia coli is necessary for nitrate/nitrite assimilation but has also been reported to either "detoxify" nitrite, or to carry out fermentative ammonification in support of anaerobic catabolism. Theoretically, nitrite detoxification would be important for anaerobic growth on nitrate, during which excess nitrite would be reduced to ammonium. Fermentative ammonification by Nir would be important for maximization of non-respiratory ATP production during anaerobic growth in the presence of nitrite. Experiments reported here were designed to test the potential role of Nir in fermentative ammonification directly by growing E. coli along with mutant strains lacking Nir or the respiratory nitrite reductase (Nrf) under anaerobic conditions in defined media while monitoring nitrogen utilization and fermentation metabolites. To focus on the role of Nir in fermentative ammonification, pH control was used in most experiments to eliminate nitrite toxicity due to nitric acid formation. Our results demonstrate that Nir confers a significant benefit during fermentative growth that reflects fermentative ammonification rather than detoxification. We conclude that fermentative ammonification by Nir allows for the energetically favorable fermentation of glucose to formate and acetate. These results and conclusions are discussed in light of the roles of Nir in other bacteria and in plants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xiaoguang</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Roy J Carver Department of Biochemistry Biophysics and Molecular Biology, Iowa State University, 4116 Molecular Biology Building, Ames, IA, 50011, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tamiev</LastName><ForeName>Denis</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Roy J Carver Department of Biochemistry Biophysics and Molecular Biology, Iowa State University, 4116 Molecular Biology Building, Ames, IA, 50011, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alagurajan</LastName><ForeName>Jagannathan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Roy J Carver Department of Biochemistry Biophysics and Molecular Biology, Iowa State University, 4116 Molecular Biology Building, Ames, IA, 50011, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>DiSpirito</LastName><ForeName>Alan A</ForeName><Initials>AA</Initials><AffiliationInfo><Affiliation>Roy J Carver Department of Biochemistry Biophysics and Molecular Biology, Iowa State University, 4116 Molecular Biology Building, Ames, IA, 50011, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Phillips</LastName><ForeName>Gregory J</ForeName><Initials>GJ</Initials><AffiliationInfo><Affiliation>Department of Veterinary Microbiology & Preventive Medicine, Iowa State University, Ames, IA, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hargrove</LastName><ForeName>Mark S</ForeName><Initials>MS</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-7127-3183</Identifier><AffiliationInfo><Affiliation>Roy J Carver Department of Biochemistry Biophysics and Molecular Biology, Iowa State University, 4116 Molecular Biology Building, Ames, IA, 50011, USA. msh@iastate.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>721 04 38</GrantID><Agency>Iowa State College of Liberal Arts and Sciences</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>11</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Arch Microbiol</MedlineTA><NlmUniqueID>0410427</NlmUniqueID><ISSNLinking>0302-8933</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064751">Ammonium Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009573">Nitrites</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.7.-</RegistryNumber><NameOfSubstance UI="D009572">Nitrite Reductases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.7.1.4</RegistryNumber><NameOfSubstance UI="D050898">Nitrite Reductase (NAD(P)H)</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D064751" MajorTopicYN="N">Ammonium Compounds</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000693" MajorTopicYN="N">Anaerobiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="Y">Fermentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D050898" MajorTopicYN="N">Nitrite Reductase (NAD(P)H)</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009572" MajorTopicYN="N">Nitrite Reductases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009573" MajorTopicYN="N">Nitrites</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Anaerobic</Keyword><Keyword MajorTopicYN="N">Escherichia coli</Keyword><Keyword MajorTopicYN="N">Hypoxia</Keyword><Keyword MajorTopicYN="N">NiR</Keyword><Keyword MajorTopicYN="N">Nitrate</Keyword><Keyword MajorTopicYN="N">Nitrite</Keyword><Keyword MajorTopicYN="N">Nitrite reductase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>06</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>10</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>10</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>11</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>6</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>11</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30406295</ArticleId><ArticleId IdType="doi">10.1007/s00203-018-1590-3</ArticleId><ArticleId IdType="pii">10.1007/s00203-018-1590-3</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Iowa</li></region><settlement><li>Ames (Iowa)</li></settlement><orgName><li>Université d'État de l'Iowa</li></orgName></list><tree><country name="États-Unis"><region name="Iowa"><name sortKey="Wang, Xiaoguang" sort="Wang, Xiaoguang" uniqKey="Wang X" first="Xiaoguang" last="Wang">Xiaoguang Wang</name></region><name sortKey="Alagurajan, Jagannathan" sort="Alagurajan, Jagannathan" uniqKey="Alagurajan J" first="Jagannathan" last="Alagurajan">Jagannathan Alagurajan</name><name sortKey="Dispirito, Alan A" sort="Dispirito, Alan A" uniqKey="Dispirito A" first="Alan A" last="Dispirito">Alan A. Dispirito</name><name sortKey="Hargrove, Mark S" sort="Hargrove, Mark S" uniqKey="Hargrove M" first="Mark S" last="Hargrove">Mark S. Hargrove</name><name sortKey="Phillips, Gregory J" sort="Phillips, Gregory J" uniqKey="Phillips G" first="Gregory J" last="Phillips">Gregory J. Phillips</name><name sortKey="Tamiev, Denis" sort="Tamiev, Denis" uniqKey="Tamiev D" first="Denis" last="Tamiev">Denis Tamiev</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30406295" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a DetoxFungiV1
| This area was generated with Dilib version V0.6.38. |  |